µL
%
Protocol The reaction this run uses. Each DNA part type has a nominal volume at a nominal concentration, which sets the mass it delivers; the reagents are the fixed volumes per reaction. Duplicate it to keep a second recipe with different enzymes or buffers.
Dosing
ng
: 1 molar
g/mol · bp
Fallback volumes — used in mass mode, or when a part has no length
Reagents — fixed volume per reaction
Drop .gb / .fasta files to add parts
Parts volume = mass ÷ measured concentration Every plasmid you might use. Give a measured concentration and the volume is the one that delivers the protocol's mass; leave it blank and the protocol's nominal volume is used.
| Part | Role | ng/µL | Length bp | on hand µL | Use | Mass |
|---|
A blank concentration means the protocol volume is used as written.
Assemblies one reaction per tube One reaction per tube. Pick its acceptor, its gene-containing plasmid and its modules, and the recipe lists every volume plus the water that brings the tube to the reaction volume.
Reaction summary what goes on the bench Every assembly in one list: module count, DNA volume, water and reaction volume per tube, with the run totals.
Master mix shared reagents The reagents every reaction shares, multiplied by the number of tubes plus an excess. Water joins the mix when every tube needs the same amount, and so does any part that appears in all of your assemblies.
tubes
%
Part usage How much of each part every assembly asks for in total, set against how much of that prep you have on hand.
Quick dilute Stock concentration, volume of stock, target concentration and final volume. Fill three and leave one empty; the empty one is worked out, along with the stock and water to combine.
ng/µL
µL
ng/µL
µL
Leave one box empty — that's the one worked out.